LOCUS Exported 4074 bp DNA circular SYN 10-FEB-2019 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4074) AUTHORS . TITLE Direct Submission JOURNAL Exported Oct 21, 2021 from SnapGene 5.3.2 https://www.snapgene.com FEATURES Location/Qualifiers source 1..4074 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(239..307) /label=LacZ alpha primer_bind 378..395 /label=M13-fwd protein_bind 432..673 /label=PB-ITR misc_feature 688..919 /label=Core Insulator misc_feature 1241..1471 /label=Core insulator protein_bind 1483..1795 /label=PB-ITR /label=PB-ITR(1) primer_bind complement(1849..1869) /label=M13-rev misc_binding complement(1875..1897) /label=LacO rep_origin complement(2237..2919) /direction=LEFT /label=ColE1 origin CDS complement(3017..3676) /label=AmpR ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ggagatcggt acttcgcgaa 421 tgcgtcgaga tttaacccta gaaagataat catattgtga cgtacgttaa agataatcat 481 gcgtaaaatt gacgcatgtg ttttatcggt ctgtatatcg aggtttattt attaatttga 541 atagatatta agttttatta tatttacact tacatactaa taataaattc aacaaacaat 601 ttatttatgt ttatttattt attaaaaaaa aacaaaaact caaaatttct tctataaagt 661 aacaaaactt ttatagaatt ctatctagag ggacagcccc cccccaaagc ccccagggat 721 gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct ccgctccggt 781 ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg gcacggggaa 841 ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc 901 tggggggata cggggaaaag gcctccaagg ccagcttccc acaataagtt gggtgaattt 961 tggctcattc ctcctttcta taggattgag gtcagagctt tgtgatggga attctgtgga 1021 atgtgtgtca gttagggtgt ggaaagtccc gcgatcgcta gcttaagcgc tgatcaattg 1081 gcgcgccgaa ttcgttaaca agctttaatt aacgcgtata cctaggatcc ggccggcctg 1141 caggtgtcct cacaggaacg aagtccctaa agaaacagtg gcagccaggt ttagccccgg 1201 aattgactgg attccttttt tagggcccat tggtatggct ttttccccgt atccccccag 1261 gtgtctgcag gctcaaagag cagcgagaag cgttcagagg aaagcgatcc cgtgccacct 1321 tccccgtgcc cgggctgtcc ccgcacgctg ccggctcggg gatgcggggg gagcgccgga 1381 ccggagcgga gccccgggcg gctcgctgct gccccctagc gggggaggga cgtaattaca 1441 tccctggggg ctttgggggg gggctgtccc ttagcggccg ctgatatcta taacaagaaa 1501 atatatatat aataagttat cacgtaagta gaacatgaaa taacaatata attatcgtat 1561 gagttaaatc ttaaaagtca cgtaaaagat aatcatgcgt cattttgact cacgcggtcg 1621 ttatagttca aaatcagtga cacttaccgc attgacaagc acgcctcacg ggagctccaa 1681 gcggcgactg agatgtccta aatgcacagc gacggattcg cgctatttag aaagagagag 1741 caatatttca agaatgcatg cgtcaatttt acgcagacta tctttctagg gttaaatcgg 1801 atgccgggac cgacgagtgc agaggcgtgc aagcgagctt ggcgtaatca tggtcatagc 1861 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 1921 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 1981 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 2041 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 2101 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 2161 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 2221 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 2281 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 2341 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 2401 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 2461 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 2521 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 2581 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 2641 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 2701 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 2761 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 2821 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 2881 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 2941 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 3001 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 3061 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 3121 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 3181 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 3241 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 3301 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 3361 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 3421 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 3481 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 3541 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 3601 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 3661 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 3721 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 3781 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 3841 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 3901 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 3961 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca 4021 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc //